An effective protocol for clonal micropropagation of Allium regelianum was optimized, including three following steps. In the first step bulbs-donors of explants were obtained from ripe seeds; in the second step – propagation under effects of exogenous cytokinins; and in the third step – final bulblets formation on the hormone-free medium. The second and the third steps may be repeated more than once. The importance of apex destruction for micropropagation was demonstrated.