Cite this article as:
Polukonova N. V., Navolokin N. А., Polukonova А. V., Mudrak D. А., Andreeva А. А., Avramets О. А., Prilepsky А. Y. INVESTIGATION OF CYTOTOXIC AND CYTOSTATIC ACTIVITY OF FLAVONOID-CONTAINING EXTRACT OF KIRKAZONE OF LOMONOSOVIDE (ARISTOLOCHIA CLEMATITIS L.) IN EXPERIMENTS IN VITRO AND IN VIVO. Izvestiya of Saratov University. New series. Series: Earth Sciences, 2018, vol. 16, iss. 2, pp. 23-?. DOI: https://doi.org/ 10.18500/1682-1637-2018-2-23-38
INVESTIGATION OF CYTOTOXIC AND CYTOSTATIC ACTIVITY OF FLAVONOID-CONTAINING EXTRACT OF KIRKAZONE OF LOMONOSOVIDE (ARISTOLOCHIA CLEMATITIS L.) IN EXPERIMENTS IN VITRO AND IN VIVO
Extract of folia and flores Aristolochia clematitis contains flavonoids and non-toxic. The extract has cytotoxic and cytostatic activity in normal animal cells. At concentrations of 15 mg/ml and higher, the extract results in 100% cell death of the Spev line. The number of dead cells of the Spev line as a whole increases with increasing concentration of the extract. The LC50 of the Kirkason extract was determined by the probit analysis method. LC50=7.24 mg/ ml. With an increase in the concentration of the extract, a marked decrease in the cell’s polymeric activity was observed. After 48 hours, the inhibition of the proliferative activity of the Spev cells is more pronounced than 24 hours later. The percentage of the Spev cells killed by the Kirkzona extract becomes smaller with increasing exposure time. The mass of the transplanted tumor of rats practically did not differ from the control group without exposure. The extract showed no antitumor activity against transplantable liver cancer of rats PC-1 in an in vivo experiment.
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The work was supported by the Ministry of Health of the Russian Federation, state task AAAA-A18-118030590040-9 “Study of extracts of medicinal plants containing flavonoids and their fractions for the purpose of creating drugs that possess antitumor, antioxidant, antiexchic and other activity”
